Coding

Part:BBa_K2083004:Design

Designed by: Virginia Massa   Group: iGEM16_WPI_Worcester   (2016-10-14)


ADAR1 2XTEN


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 98
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1017


Design Notes

We preformed codon optimization to get rid of any EcoRI, XbaI, SpeI, and PstI sites.


Source

This part is derived from the sequence of human ADAR1.

References